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BACTERIOLOGY
and LABORATORY PROCEDURES Course on CD
CD
Contents: -----
267 pages
BACTERIOLOGY and
LABORATORY PROCEDURES Course on CD The whole purpose of
clinical laboratory procedures is to provide the
clinician doing diagnostic work with specific
information needed to round out his picture of
the disorders he has observed in the patient.
Clinical bacteriology can contribute its part by
supplying data about the microscopic life
involved and the susceptibility of such life to
particular drugs. To identify bacterial growth,
you must take certain steps that will enable you,
through a process of elimination, to choose the
microscopic form that fits the findings you have
obtained.
Steps that are often essential include:
1. Observing the type of growth when first
isolated on culture media.
2. Making a microscopic examination on stained
material from an isolated culture of that colony.
3. Performing various tests to obtain a list of
the characteristics of the organism.
4. Making a complete identification of the
organism.
The course instructional material consists of six
lessons as follows:
- Lesson 1, Introduction.
- Lesson 2, Microscopic Examination and
Cultivation of Bacteria.
- Lesson 3, Common Tests and Gram-Positive Cocci.
- Lesson 4, Gram-Negative Cocci; Gram-Positive
Bacilli.
- Lesson 5, Enterobacteriaceae.
- Lesson 6, Other Pathogenic Gram-Negative
Bacilli and Antibiotic Sensitivity Tests.
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BACTERIOLOGY
and LABORATORY PROCEDURES Course
on CD
TABLE OF
CONTENTS
1 INTRODUCTION
Section I. Host-Parasite Relationships.
1-1--1-3
Section II. Aspects of Pathogenicity.
1-4--1-6
Section III. Host Resistance . 1-7--1-8
Section IV. The Bacterial Cell. 1-9--1-14
Section V. Collecting and Processing of
Bacteriological
Specimens . 1-15--1-22
Exercises
2 MICROSCOPIC EXAMINATION AND CULTIVATION
OF BACTERIA
Section I. Microscopic Examination of
Bacteria . 2-1--2-12
Section II. Cultivation of Bacteria (Media) .
2-13--2-15
Section III. Environmental Factors .
2-16--2-18
Section IV. Anaerobic Methods . 2-19--2-24
Section V. Antibacterial Agents,
Sterilization, and
Aseptic Technique. 2-25--2-34
Section VI. Isolation of Bacteria. 2-35--2-39
Exercises
3 COMMON TESTS AND GRAM-POSITIVE COCCI
Section I. Common Tests. 3-1--3-14
Section II. Gram-Positive Cocci:
Staphylococci . 3-15--3-19
Section III. Gram-Positive Cocci:
Streptococci. 3-20--3-26
Section IV. Gram-Positive Cocci: Pneumococci
. 3-27--3-32
Exercises
4 GRAM-NEGATIVE COCCI AND GRAM-POSITIVE
BACILLI
Section I. Introduction . 4-1--4-2
Section II. Neisseria gonorrhoeae. 4-3--4-17
Section III. Neisseria meningitides .
4-18--4-21
Section IV. Gram-Positive Bacilli:
Corynebacteria
and Related Species . 4-22--4-28
Section V. Gram-Positive Bacilli: Bacillus
Species . 4-29--4-33
Section VI. Gram-Positive Bacilli: Clostridia
. 4-34--4-38
Section VII. Gram-Positive Bacilli:
Mycobacteria. 4-39--4-50
Exercises
5 ENTEROBACTERIACEAE
Section I. Introduction . 5-1--5-3
Section II. Pathogenicity of
Enterobacteriaceae. 5-4--5-9
Section III. Enteric Media . 5-10--5-16
Section IV. Preliminary Screening of Cultures
for
Enterobacteriaceae. 5-17--5-19
Section V. Identification of Enteric
Organisms. 5-20--5-35
Exercises
6 OTHER PATHOGENIC GRAM-NEGATIVE
BACILLI AND ANTIBIOTIC SENSITIVITY TESTS
Section I. Pseudomonas . 6-1--6-3
Section II. Brucella . 6-4--6-7
Section III. Haemophilus . 6-8--6-10
Section IV. Bordetella. 6-11--6-12
Section V. Francisella Tularensis .
6-13--6-16
Section VI. Miscellaneous Organisms .
6-17--6-18
Section VII. Antibiotic Sensitivity Tests.
6-19--6-23
Exercises
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